| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000910 |
| Chromosome: | chromosome 12 |
| Location: | 8764072 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547300 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAAGTTGTTGGCGCTAGCTGACTCATGTGCAACTACTTAAAAGCCGT |
| Internal bar code: | CAGAAAATCACCCGTTGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 446 |
| LEAP-Seq percent confirming: | 21.7391 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTCGGCCCCATGCTACATT |
| Suggested primer 2: | GTTCCGAAAATGATCCGGCG |