Insertion junction: CLIP2.000911_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000911
Chromosome:chromosome 2
Location:3326441
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g097800 HLA3,MRP1 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase; Bicarbonate ABC transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGGCGACCATGGCAGGGGCAGGGAAGTGCAGCAATTTCCGAGTGGGC
Internal bar code:GGTGTCTAATAATTTCCAGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1677
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AACATGGCCTGAGAAGGTCG
Suggested primer 2:GTGGAGTGGAGCGAAAGTGA