| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000918 |
| Chromosome: | chromosome 12 |
| Location: | 4036232 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516300 | CGL154 | Conserved in the Green Lineage; (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACATAACGTTCAAGGTGAGCGCAACCACGCAACAGACGTCAAGGGGT |
| Internal bar code: | TTGAGTCGACTGGAATGTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2428 |
| LEAP-Seq percent confirming: | 90.7407 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGGTGTTGAGATTGCG |
| Suggested primer 2: | TGCTGCACCGGTATCAACTT |