| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000927 |
| Chromosome: | chromosome 12 |
| Location: | 6298723 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCTCCGCACCCGCGTGCGAGGGCGCCGCCGCGCTGCACCTGCCTCGC |
| Internal bar code: | GGCCATGGTTCGTTGATTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAGCTCGTCAGGCTGTT |
| Suggested primer 2: | GCTATCTCTGTGCGCGTTTG |