| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000934 |
| Chromosome: | chromosome 3 |
| Location: | 4130473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172700 | outside_mRNA | ||
| Cre03.g172750 | (1 of 1) IPR002893//IPR012677 - Zinc finger, MYND-type // Nucleotide-binding alpha-beta plait domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGAGCTCGTTCATGGCAGTAGTACATGGCCGCAGAGCAACACCTTAG |
| Internal bar code: | AATCTAGAATCTATGAAGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2032 |
| LEAP-Seq percent confirming: | 97.8723 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCCGCGATTGGATATGT |
| Suggested primer 2: | CACACGAGTCTGCAGGAGTT |