| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000946 |
| Chromosome: | chromosome 3 |
| Location: | 4127590 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCCTGTGGCGGCGCAGCAGGGCCGGCTGCAGGGCCAGCAGCAAGGCA |
| Internal bar code: | AGGATTCCACGTGTGTGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4098 |
| LEAP-Seq percent confirming: | 42.3729 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAGCCGCTCAAACTCAG |
| Suggested primer 2: | GTGTGTCTTGTCCCTCCTCG |