Insertion junction: CLIP2.000952_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000952
Chromosome:chromosome 10
Location:340389
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g420350 PSAE,PSAE1 (1 of 1) K02693 - photosystem I subunit IV (psaE); Photosystem I reaction center subunit IV, 8.1 kDa CDS
Cre10.g420400 (1 of 1) K14289 - exportin-5 (XPO5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAACATCGCGGGTGCGTGATCGGCAAACTCCGAATCATGTCGCCGCCC
Internal bar code:TGTATTCAGCATCGTTACGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1674
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGATCGCCAAGTCTATGC
Suggested primer 2:AGAGTTGCTGGAAACCGAGG