| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000957 |
| Chromosome: | chromosome 6 |
| Location: | 2967594 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273750 | SABC1,SABC,SUA1 | Chloroplast sulfate transporter; (1 of 1) K02045 - sulfate transport system ATP-binding protein (cysA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCACGAAAGCCCGACACTCGGTACCCCTCCCAAAAGCTGAAAAATGG |
| Internal bar code: | TAGGGCTCGGGTGGAGGAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1433 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTTGCAATGCAGTCCAGC |
| Suggested primer 2: | TTGGGACTCCAGCGAAACTC |