| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000960 |
| Chromosome: | chromosome 3 |
| Location: | 5938731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g188950 | SMM11 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PF07942 - N2227-like protein (N2227) | 3'UTR |
| Cre03.g189000 | RAD54A,SRH8 | SNF2-related DNA/RNA helicase; (1 of 1) PTHR10799//PTHR10799:SF607 - SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY-RELATED // SNF2-RELATED DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCTTAAAAGAAAATCCTGATCACGTCCATGCTTTGCGCTGCGGGTGCC |
| Internal bar code: | TTTAACATGTATCTTCCTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1367 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATTGAGTTGTCGCTGGA |
| Suggested primer 2: | GGTCACCTGCATCAACCAGA |