Insertion junction: CLIP2.000972_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.000972
Chromosome:chromosome 7
Location:514728
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g316050 CDJ2 Chloroplast DnaJ-like protein 2; (1 of 4) K03686 - molecular chaperone DnaJ (dnaJ) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGAACCGCACGCATCCATTGAATCTGCTGCTTGACCTTTGTAATGAC
Internal bar code:AACGAGATCGAATAGAACATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCGGCTCTTTGCTTTGTTGA
Suggested primer 2:GGTGGTTGATGTGGCGAGTA