Insertion junction: CLIP2.000986_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000986
Chromosome:chromosome 13
Location:3851402
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g589450 FBB6L1 (1 of 1) IPR002035//IPR013694 - von Willebrand factor, type A // VIT domain; FBB6 like protein 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACGGTCATGTTGAAGCGCACGCCGCTGTCCACGGGCAGGTCACGAAC
Internal bar code:TGCTAGGGTATACCGGGCTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4248
LEAP-Seq percent confirming:85.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGACCTACGTCCTCTCCT
Suggested primer 2:CAGGCTCAGAGCTAACCACC