| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000989 |
| Chromosome: | chromosome 10 |
| Location: | 2233409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433750 | PAP1 | Class-I Nucleotidyl-transferase 1, nuclear; (1 of 1) K14376 - poly(A) polymerase (PAP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGGTTCCGGGTTGGCAAAGGTTTGTTTTGGGATCGGAGTGAACTACC |
| Internal bar code: | ACCCGGATCAGTATGGCTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2614 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGGATTGCTCTTCATG |
| Suggested primer 2: | CAATTCAATACCTGCGCCCG |