Insertion junction: CLIP2.000999_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000999
Chromosome:chromosome 13
Location:2724209
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g581900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCATCGGAGTCCCGTTCGAAGCTCAGGTCGCGCTTCTGCTGCTGCGGA
Internal bar code:AAGTGTCGCTTGAGCTAAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1962
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGACGCTAGGGTGGAGC
Suggested primer 2:AGTGTTCCCCGATGCTTCAG