Insertion junction: CLIP2.001048_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001048
Chromosome:chromosome 6
Location:2972927
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273750 SABC1,SABC,SUA1 Chloroplast sulfate transporter; (1 of 1) K02045 - sulfate transport system ATP-binding protein (cysA) gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAACTATCCCGCGCGACTGAACACTCTATAGTACTTCAATTGTCAGAAT
Internal bar code:GTAGATCGGTGTAACGTGCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2142
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGGTGTGTCACTTCCTTA
Suggested primer 2:TGTGTGGCAAGGCTATCTGG