Insertion junction: CLIP2.001092_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001092
Chromosome:chromosome 3
Location:5195026
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g181500 DPE1,STA11 Disproportionating enzyme 1; (1 of 1) PTHR32438:SF5 - 4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGCACCAGCGGCAGCAGCTGCCAGCACTGCATGCCGTGGTCGGCGAG
Internal bar code:CCTAAGTTAGTGGACCGTCTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1476
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CACATGAGCGCGTGTACATG
Suggested primer 2:GTCGCCGATGAGTTTGATGC