| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.001128 |
| Chromosome: | chromosome 3 |
| Location: | 9152005 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200319 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACTGGGACCAGCACCAGGCACCTGGTCACCTGCACCTGCGGCAACAAC |
| Internal bar code: | ACGTAGCGCGCCGACAGTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1652 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGCTTCCAGGAATGACT |
| Suggested primer 2: | GTCGATAGCAAGCGGGATGA |