Insertion junction: CLIP2.001132_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001132
Chromosome:chromosome 7
Location:2194472
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327100 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGATCCAGGGCTGCGACGGGCGCTGTTTGGAACCGTGCTGAGTAACGG
Internal bar code:GTCCATTATGGTCGCGATACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:371
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGGATGGCATAATGACGA
Suggested primer 2:CCTTCCACCCCACAGAATCC