| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001134 |
| Chromosome: | chromosome 1 |
| Location: | 520773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003150 | protein of unknown function | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTAAAAGGCGAAAGAGATATTCGCTACGCGTTCAGCTCACCGCGGATA |
| Internal bar code: | GTTTAGGAGATGGGGTGAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 80.5556 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACAAATGCACACACAGT |
| Suggested primer 2: | CAAACATGAGGCGGCGTATG |