Insertion junction: CLIP2.001138_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001138
Chromosome:chromosome 2
Location:585943
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g077150 CPLD14 Conserved in the Plant Lineage and Diatoms; (1 of 2) PF05684 - Protein of unknown function (DUF819) (DUF819) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCCTAGCTGGAGTGGGCGCGGCTGACAGCCATACTGCTTCGCACATCA
Internal bar code:TTCCTGCATTTATGTTGTCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1232
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TCAACTACATGGCCGTGTCC
Suggested primer 2:TGGGTCGTTACAGTCGGTTG