| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001144 |
| Chromosome: | chromosome 2 |
| Location: | 583639 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077100 | GSH1 | (1 of 1) 6.3.2.2 - Glutamate--cysteine ligase / Gamma-glutamylcysteine synthetase; Gamma-glutamylcysteine synthetase | 3'UTR |
| Cre02.g077150 | CPLD14 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF05684 - Protein of unknown function (DUF819) (DUF819) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGCTCAATCTAAAACAATACAACTCCTGCGGGCATTCGAGCTGAAAC |
| Internal bar code: | CAAATTGGAGGAGCTGAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1237 |
| LEAP-Seq percent confirming: | 70.7692 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAAGAGGTGAGCTGAACT |
| Suggested primer 2: | TTGCGGTGGGATGCATAGTT |