Insertion junction: CLIP2.001146_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001146
Chromosome:chromosome 12
Location:8558378
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548950 LHCBM7 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGTTCGCATATCCGAGCAAGGATGGCCAGAGATCCGCACTGCTGGCGT
Internal bar code:CGAGTAATATTTTGAAATGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3250
LEAP-Seq percent confirming:75.1724
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:145

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGAGACATCCATCCACG
Suggested primer 2:AAATTTAGGCCGAGGGGGTG