| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.001168 |
| Chromosome: | chromosome 17 |
| Location: | 3533293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724600 | PAO2,TIC55-2 | Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) | 5'UTR |
| Cre17.g724650 | (1 of 9) PTHR13789//PTHR13789:SF186 - MONOOXYGENASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACGGCGTTGATCTGCCGGTTTGCAAATGTTGCGCTGCATCAGCAACCC |
| Internal bar code: | CCCGTGATTTTGGCCTAAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3889 |
| LEAP-Seq percent confirming: | 19.3548 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCGACTCCCAAACCTAGG |
| Suggested primer 2: | CCACCCCCAAGTATCACCAC |