| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.001184 |
| Chromosome: | chromosome 5 |
| Location: | 110690 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242178 | (1 of 1) PTHR23344//PTHR23344:SF10 - GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATTACTTGTTGCCTGTACTTGTGCGTTTGTCTGTGCACACAAGTAA |
| Internal bar code: | TCGAAGAACGTTTTAGCATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTCCACATAGCGCAGG |
| Suggested primer 2: | ATAGGACCCAGCTCGTCACT |