| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.001231 |
| Chromosome: | chromosome 3 |
| Location: | 1941333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155250 | (1 of 1) IPR002202//IPR029058 - Hydroxymethylglutaryl-CoA reductase, class I/II // Alpha/Beta hydrolase fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCGGAAGGAACCGGCCGATGTGCGAGGGGGCGGTTGGATAAGGTGA |
| Internal bar code: | CAAAGTTAGGCGGGTGCGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2375 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGCACAGGTCAAAACAC |
| Suggested primer 2: | GGACATGACAGACAGTGGGG |