| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001250 |
| Chromosome: | chromosome 9 |
| Location: | 1608180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398700 | CFA2,CPLD27 | (1 of 1) 2.1.1.140 - (S)-coclaurine-N-methyltransferase; coclaurine N-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTGTACATGGGTGGCCGGGGGCGCGGCGGGGCGGGGCTGCAAGGCA |
| Internal bar code: | GAGGTTGAGCCCGAAGTCTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1818 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACCACTACCTGGACTTT |
| Suggested primer 2: | TCTGTGTGTGCCTGTGTGTT |