Insertion junction: CLIP2.001265_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001265
Chromosome:chromosome 3
Location:2099195
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g156500 (1 of 1) IPR000104//IPR016084 - Antifreeze protein, type I // Haem oxygenase-like, multi-helical intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTTTGTGCTATCCTGCAGATCCGAGGCATGACTTCGGATTTTGTCACA
Internal bar code:TAGTCGTCTTTATCGGGTACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2800
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTCAGTTTGCGCAAGAA
Suggested primer 2:CAGTGCACAAACACACCTCG