Insertion junction: CLIP2.001271_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001271
Chromosome:chromosome 2
Location:587880
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g077150 CPLD14 Conserved in the Plant Lineage and Diatoms; (1 of 2) PF05684 - Protein of unknown function (DUF819) (DUF819) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTACTCTCGACTCTCACAGCTCCACACGCATCGCATATACTTCACAAC
Internal bar code:TGTGGCCTTCGATGGGTGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:4.25532
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTGTCTTGCTCATCCACC
Suggested primer 2:CAAGTACGTGTCGGAACCCA