Insertion junction: CLIP2.001302_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.001302
Chromosome:chromosome 1
Location:8134112
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre01.g066917 LHCBM1 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTATGCTCCTCGTGCCCGCTCCACAGGTCCGACCACCTGGCCAACCCCG
Internal bar code:AAATGACCTCCGGCGTACATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1860
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCTTCGGTTCGCTCGGA
Suggested primer 2:AAGCCTTCAGGACATGGAGC