| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001303 |
| Chromosome: | chromosome 8 |
| Location: | 2251261 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370550 | (1 of 1) 1.1.99.39 - D-2-hydroxyglutarate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCCCTCTTCTGCTTTGCTTCGCCTTGTTCTTGCCCCTGCTTGTCACA |
| Internal bar code: | TACGGACTGTGTAGTTTTGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1603 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTTCAACCTGCACAAAT |
| Suggested primer 2: | GACCAGTCCCATTTCGCTGA |