Insertion junction: CLIP2.001309_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001309
Chromosome:chromosome 11
Location:773321
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467631 CGL90 Zinc carboxypeptidase; Conserved in green lineage; (1 of 1) 3.4.17.15 - Carboxypeptidase A2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCACGAGAGAGACGACAACAAACGGCAGCCAGCTTTGCAAGCCTGCCA
Internal bar code:CACCGCCAATCGTTGGGTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1442
LEAP-Seq percent confirming:41.4286
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:AATGTGTGCGACGTACCAGT
Suggested primer 2:TCCAGGTGTGAGGACCGTAT