| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001325 |
| Chromosome: | chromosome 13 |
| Location: | 164850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g563150 | PAB1,CGLD8 | Assembly factor for CF1 or F1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGCCGCCGCCAGCTGCTCCCGGATGCCGTCGTAGCCGCCCTCCGCCT |
| Internal bar code: | CATTACAGGAAATTCGCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCACACACACACATACA |
| Suggested primer 2: | ACGACCACTTCATCAAGCGT |