Insertion junction: CLIP2.001330_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.001330
Chromosome:chromosome 9
Location:3502646
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g396289 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACATCACTGGCCACGGTTGGCTGAATTTGACACGGGAAATGACGACCCC
Internal bar code:CGGTCGTTTCTCTGGTATGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:70.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTGCTTTACACACGCAC
Suggested primer 2:GCGCAAGAAAGGAAACGTGT