| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001335 |
| Chromosome: | chromosome 12 |
| Location: | 7216550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559050 | (1 of 1) PTHR24006//PTHR24006:SF22 - FAMILY NOT NAMED // BCDNA.LD22910 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCCTAGCAATAATCGGACCCCATGCACCGCTCATCACACCTCGCGCT |
| Internal bar code: | TTGTAATAAGATTTTGACAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 54.2373 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATTGCGGGTGGAAAAG |
| Suggested primer 2: | AGGTGCCCTGCGTCATTTAA |