Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.001344 |
Chromosome: | chromosome 2 |
Location: | 6488134 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g388750 | TEF21 | (1 of 1) PTHR11200:SF170 - PHOSPHOINOSITIDE PHOSPHATASE SAC1; Phosphoinositide phosphatase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCCGGGGCACGGCCGCGGTAAGCACGCCGACCCAAACCCGGGCG |
Internal bar code: | GGGGGGTGTCGGATATAGGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2250 |
LEAP-Seq percent confirming: | 62.3188 |
LEAP-Seq n confirming: | 43 |
LEAP-Seq n nonconfirming: | 26 |
LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGCTGCTCCATTTTCAACA |
Suggested primer 2: | CAGTCGTTATCAAAGCGCCG |