| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001360 |
| Chromosome: | chromosome 10 |
| Location: | 1941853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431850 | PGM18 | Phosphoglycerate mutase family protein; (1 of 1) PTHR23029//PTHR23029:SF49 - PHOSPHOGLYCERATE MUTASE // PHOSPHOHISTIDINE PHOSPHATASE SIXA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACCAACCTGTGCCGCCTGCTGCCGGCCTTGGTCCGAGACCGGTCGAT |
| Internal bar code: | GGAAAAACGAAGTGATATGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 451 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCACGTTGACCACTGCT |
| Suggested primer 2: | TACCGTAATAGTGCGCGTCC |