| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001391 |
| Chromosome: | chromosome 5 |
| Location: | 1580151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234750 | CGLD25 | Conserved in the Green Lineage and Diatoms | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTGGCGTGTGGCACTACCGTGCGCCGCAGAACCGGACATGCGGCCT |
| Internal bar code: | AGACTTAGTTCGGGTACGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2869 |
| LEAP-Seq percent confirming: | 86.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCGTGTTCCTTTTGGG |
| Suggested primer 2: | GGGTCGTTCACGGTTTTGTG |