| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.001392 |
| Chromosome: | chromosome 17 |
| Location: | 4085020 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728850 | RAA15 | component psaA trans-splicing sub complex II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCCTCACGCATCCCGCATGCCTGATGCATAACATGCGGCGCGCGGGT |
| Internal bar code: | GCTGCGAGCCTGAGATGGATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTGGAACCGCATGTTT |
| Suggested primer 2: | AACATGCACGCTGTCAAACC |