| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.001401 |
| Chromosome: | chromosome 13 |
| Location: | 125916 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g562750 | CGLD38 | Conserved in the Green Lineage and Diatoms; (1 of 1) PF14234 - Domain of unknown function (DUF4336) (DUF4336) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCAGGGAGCTGGGTTAATATCATGTGTAAGGCGTTGCACTGGCGCAC |
| Internal bar code: | AGCTCCGATATCCGGTTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1124 |
| LEAP-Seq percent confirming: | 67.6471 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGTTGTTCAGGCTGTTG |
| Suggested primer 2: | GGGGCCGGACGAAATAAAGA |