| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.001414 |
| Chromosome: | chromosome 10 |
| Location: | 4073405 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448051 | SFL1,CPSFL1 | (1 of 1) PTHR10174:SF156 - SEC14P-LIKE PHOSPHATIDYLINOSITOL TRANSFER FAMILY PROTEIN; CRAL-TRIO hydrophobic ligand-binding (Sec14) domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCAATCAGGTACGCCGCCAGGCGCTTGGAGCCGTCGATAGGGTACTCG |
| Internal bar code: | ATCATTCGTTTGGACGTATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 182 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGCTCAAGTGGTACCTG |
| Suggested primer 2: | GTCGCTAGAATCCGTCCCAG |