| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.001425 |
| Chromosome: | chromosome 3 |
| Location: | 4341950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174525 | CDS | ||
| lncRNA_TCONS_00065772 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCGCCTGCTTACCCTCGCAACTTCGCCAGCTTCGGCAGCCTCGGCT |
| Internal bar code: | GCAAGTGTGCCAAGTGGATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1253 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCCCCTCACCCATGAA |
| Suggested primer 2: | GTTCCTTCCCAGAACGCTGA |