Insertion junction: CLIP2.001434_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.001434
Chromosome:chromosome 9
Location:3061371
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393876 (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTTGCCGCTTGGGCACTTGCGCGCGGTGGTGTCCCGCGCGCGTGTGAA
Internal bar code:TTCGGAGTAACGTACTAGACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3003
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:107
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:107

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACTGAAATGGCTATGCG
Suggested primer 2:GGGTCTGGTTGTGGTTGTGA