| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.001434 |
| Chromosome: | chromosome 15 |
| Location: | 854521 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g640650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATAGAGTCAAAGTTATGGAGCGTGTGACTGACAAGTCACATAAGTCAA |
| Internal bar code: | TGTTGCGGCAGGATAGGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 78.4314 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCACGTGCGTTCAGATC |
| Suggested primer 2: | AGGCGGCCCAAAGTAATTGA |