Insertion junction: CLIP2.001437_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.001437
Chromosome:chromosome 7
Location:3848531
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g338850 3'UTR
Cre07.g338900 (1 of 1) PTHR12072//PTHR12072:SF4 - CWF19, CELL CYCLE CONTROL PROTEIN // CWF19-LIKE PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCGTGTGTGATCATGCTCACCCGCTGAGGCGGTCACCAACGCACACA
Internal bar code:AACTGCAATAGCAACGGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1083
LEAP-Seq percent confirming:71.4286
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCCACGCATAGGAGCAT
Suggested primer 2:GCGGCTATGATGAGGCTCTT