| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001446 |
| Chromosome: | chromosome 12 |
| Location: | 7264855 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558550 | CPLD60 | (1 of 1) PTHR10992:SF351 - HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN; possible hydrolase | 5'UTR |
| Cre12.g558600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCACACTTACGCTGGAACAGCTGTAAAGTTGAGCAAGCTGGAAACAT |
| Internal bar code: | AGATGCTTGTTTGTTAGTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 588 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGACCCCCAGCCATAGAT |
| Suggested primer 2: | GAATCGATGCGGGCTGAGTA |