Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.001462 |
Chromosome: | chromosome 6 |
Location: | 2982045 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g273850 | HTB7 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAGCCCAAGGCCGAGAAGGCCGCAAAGAAGGCCAAGAAGGAGCCCTCC |
Internal bar code: | TTAGTAGCGGTCAGACAGCGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3805 |
LEAP-Seq percent confirming: | 91.6129 |
LEAP-Seq n confirming: | 142 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 155 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGTTGACGAGAAGGGAACA |
Suggested primer 2: | ATTCAGCCCCAACTCACGAG |