Insertion junction: CLIP2.001467_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001467
Chromosome:chromosome 2
Location:1310200
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g082550 ZEP,ZEP1 Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCCCAGCGTGCTGGAGTGGGTGCTGGGCGGCAACACCGACCACGTGGC
Internal bar code:TGTACATGATGTCCACTCATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGAGGCGTCGTTGTTCAT
Suggested primer 2:CAACGTGGTGGACCTGATCA