| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.001484 |
| Chromosome: | chromosome 2 |
| Location: | 983698 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079600 | TSF2 | (1 of 1) PTHR11538:SF41 - PHENYLALANINE--TRNA LIGASE, MITOCHONDRIAL; Phenylalanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTACAGATCTTCGCCCGTCCTGTCCAGTGTCCACACCTTTTGAAGTG |
| Internal bar code: | ATCATAACGAGAGAAGTTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1503 |
| LEAP-Seq percent confirming: | 76.1905 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGATGAGGAGATCAACGC |
| Suggested primer 2: | CTCACACTGTGCTTGTTGCC |