Insertion junction: CLIP2.001512_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.001512
Chromosome:chromosome 1
Location:5397577
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g038050 COP7,HKR3,COP Histidine kinase rhodopsin 3; (1 of 1) PF00072//PF00512//PF01036 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGCCAGCCCGGCGGCGGCGATGGCCAGCGGCGCCAGCACACCGTGCGG
Internal bar code:TTAACTTATATCACAAAGGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2898
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCTTCCACGTAGCGGAACTG
Suggested primer 2:CTGTGTGGTGTGTGCTTTCG