Insertion junction: CLIP2.001513_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.001513
Chromosome:chromosome 7
Location:1140656
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320750 CYG12 Adenylate/guanylate cyclase, nitric oxide sensing; (1 of 5) K12319 - guanylate cyclase soluble subunit beta (GUCY1B) 3'UTR
Cre07.g320800 (1 of 1) PF09468 - Ydr279p protein family (RNase H2 complex component) (RNase_H2-Ydr279) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATTGTTACAGCTAAAGCGGGGTGACGGGCTTTGTACCGCGTGCATTG
Internal bar code:GCCATCACGAGATATATATTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4631
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:130

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCGTTGCCTTTTGCGATT
Suggested primer 2:TTGGGACGATTGGATTGCGA