| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.001523 |
| Chromosome: | chromosome 3 |
| Location: | 9061721 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202113 | (1 of 1) 6.3.2.6 - Phosphoribosylaminoimidazolesuccinocarboxamide synthase / SAICARs | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGGTCACGCCCTCTCGCGTAGCCCCTCTACATTAGTGGTGATGATGAT |
| Internal bar code: | GCGTCTATGGGCTCTACAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 463 |
| LEAP-Seq percent confirming: | 62.1622 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGTCCCACTTAACCCCC |
| Suggested primer 2: | CCTTCTGTAGGTTGGCCAGG |